N-acylamino-alpha-arylacetamido cephalosporins and antibacterial compositions and methods containing them

ABSTRACT

A cephalosporin derivative of the formula (I), ##STR1## wherein A is a mono- or polycyclic heteroaromatic ring, which contains at least one nitrogen atom as a hetero atom and which may be substituted with one or more substituents; R is a phenyl group substituted with at least one substituent selected from the group consisting of an amino group, a hydroxy group, a ureido group and a hydroxymethyl group; and X is an --OCOCH 3  group or an --S-Het group in which Het is a 5- or 6-membered heterocyclic ring containing one to four hetero atoms selected from the group consisting of nitrogen, oxygen and sulfur atoms which may be substituted with one or more substituents, and the non-toxic pharmaceutically acceptable salts thereof which are useful as an antimicrobial and prepared by the reaction of a compound of the formula (II), 
     
         ho--a--cooh                                                (ii) 
    
     wherein A is as defined above, or a reactive derivative thereof, with a compound of the formula (III), ##STR2## wherein R and X are as defined above, or a derivative thereof.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a novel cephalosporin and to a processfor the preparation thereof. More particularly, it relates to a novelcephalosporin of the formula (I), ##STR3## wherein A, R and X are asdescribed hereinafter, the non-toxic pharmaceutically acceptable saltsthereof and to the preparation thereof.

2. Description of the Prior Art

It is known that cephalosporin series compounds such as Cephalothin andCefazolin are very effective and are widely used as chemotherapeuticagents for infectious diseases caused by gram-positive or gram-negativebacteria.

However, these cephalosporin series compounds have no effect oninfectious diseases caused by Pseudomonas aeruginosa which has beenspreading increasingly in recent years and is often very difficult tocure. Cephalosporin series compounds which are effective againstPseudomonas aeruginosa are not yet commercially available.

SUMMARY OF THE INVENTION

Accordingly, in one embodiment, the present invention provides compoundsof the formula (I) ##STR4## wherein A is a mono- or polycyclicheteroaromatic ring, which contains at least one nitrogen atom as ahetero atom and which may be substituted with one or more substituents;R is a phenyl group substituted with at least one substituent selectedfrom the group consisting of an amino group, a hydroxy group, a ureidogroup and a hydroxymethyl group; and X is an --OCOCH₃ group or an --S-Het group in which Het is a 5- or 6-membered heterocyclic ringcontaining one to four hetero atoms selected from the group consistingof nitrogen, oxygen and sulfur atoms which may be substituted with oneor more substituents, and the non-toxic pharmaceutically acceptablesalts thereof which are useful as antimicrobial agents.

In another embodiment the invention provides a pharmaceuticalcomposition containing at least one compound of the formula (I) or anon-toxic pharmaceutically acceptable salt thereof (I) as an activeingredient.

In a further embodiment the invention provides a process for thepreparation of the compounds of the formula (I) and the non-toxicpharmaceutically acceptable salts thereof.

DETAILED DESCRIPTION OF THE INVENTION

In the formula (I), A is a mono- or poly-cyclic heteroaromatic ringcontaining at least one nitrogen atom as the hetero atom, and examplesinclude quinoline, isoquinoline, cinnoline, naphthyridine, quinoxaline,pyrazolopyridine, pyridopyrazine, thiazolopyrimidine, pyridopyrimidine,pyridine, pyrimidine, pyridazine, triazine, pyrazine and the like. Theseheterocyclic rings can be substituted with one or more substituentsselected from the group consisting of a halogen atom, a lower alkylgroup, a lower alkoxy group, a lower alkanoyl group, a loweralkoxycarbonyl group, a lower alkylthio group, a mercapto group, ahydroxy group, a lower alkoxymethyl group, a cyano group, a nitro group,a lower alkylsulfonyl group, an arylsulfonyl group, a sulfamoyl group, acarbamoyl group, an aryloxycarbonylamino group, an acetoacetylaminogroup, a lower alkylamino group, a lower dialkylamino group, a lowerhaloalkyl group, a lower alkenyl group, an aryl group, a cycloalkylgroup and the like in addition to the hydroxyl group.

In the formula (I), the R preferably is a substituted phenyl group ofthe formula ##STR5## wherein R₁ is a ureido group, a hydroxyl group, anamino group, or a hydroxymethyl group; R₂ and R₃ each is a hydrogenatom, a chlorine atom, a bromine atom, a fluorine atom, an iodine atom,a nitro group, a di-lower alkylamino group, a lower alkanoylamino group,an amino group, a hydroxyl group, a lower alkanoyloxy group, a loweralkyl group, a lower alkoxy group, a trifluoromethyl group, ahydroxymethyl group, a sulfamyl group or the like.

In the formula (I), X is an --OCOCH₃ group or an --S-Het group wherein--Het is a five- or six-membered heterocyclic ring containing one tofour hetero atoms selected from the group consisting of nitrogen, oxygenand sulfur atoms. The heterocyclic ring may be substituted with a (C₁-C₄)alkyl group, a hydroxy group, a lower alkoxy group, a mercapto groupor a hydroxymethyl group. Examples of suitable heterocyclic ringsinclude, for example, 1-methyl-tetrazol-5-yl,2-methyl-1,3,4-thiadiazol-5-yl, 1,2,3-triazol-5-yl, 1,2,4-triazol-3-yl,tetrazol-5-yl, 2-methyl-tetrazol-5-yl, 2-mercapto-1,3,4-thiadiazol-5-yl,2-hydroxymethyl-1,3,4-oxadiazol-5-yl,2-hydroxymethyl-1,3,4-thiadiazol-5-yl,2-aminomethyl-1,3,4-thiadiazol-5-yl, 3-hydroxypyridazin-6-yl,1,3,4-thiadiazol-5-yl, 2-methylamino-1,3,4-thiadiazol-5-yl and the like.

Examples of non-toxic pharmaceutically acceptable salts derived from thecompounds of formula (I) include the sodium salt, the potassium salt,the calcium salt, the magnesium salt, the triethylamine salt, thediethanolamine salt, the morpholine salt, the procaine salt, theL-arginine salt, the L-lysine salt and the like.

The α-carbon atom of the side chain (phenylglycine moiety) attached tothe 7-position of the formula (I) is an asymmetric carbon atom andtherefore two optically active isomers exist. These two isomers(D-diastereomer and L-diastereomer) and the DL-form are included withinthe scope of the present invention, but the D-diastereomer is preferred.

As used herein, the term "lower alkyl" preferably means a straight orbranched alkyl group or alkyl moiety having one to four carbon atoms.

The term "lower alkoxy" preferably means a straight or branched alkoxygroup or moiety having one to four carbon atoms.

The term "lower alkanoyl" preferably means an alkanoyl group or moietyhaving two to five carbon atoms.

The term "halogen" means fluorine, chlorine, bromine, and iodine.

The term "aryl" preferably includes a phenyl group and a pyridyl groupand the term "arylsulfonyl" preferably includes a phenylsulfonyl groupand a pyridylsulfonyl group.

The term "aryloxycarbonylamino" preferably includes aphenoxycarbonylamino group.

The term "haloalkyl" means a halogen-substituted alkyl group having oneto four carbon atoms.

The term "lower alkenyl" preferably includes an alkenyl group having upto four carbon atoms.

The term "cycloalkyl" means a cycloalkyl group having three to sixcarbon atoms.

The compound of the formula (I) of the present invention can be preparedby reacting a carboxylic acid of the formula (II),

    ho--a--cooh                                                (ii)

wherein A is as defined above, or a reactive derivative thereof, with acompound of the formula (III) ##STR6## wherein R and X are as definedabove, or a salt or derivative thereof, and when X is an --OCOCH₃ group,by further reacting the resulting product with a compound of theformula, SH-Het in which Het is as defined above, to convert X into an--S-Het group, if necessary.

Referring more particularly to the process, inert solvents which can beused in the reaction between the compounds of the formulas (II) and(III) include polar solvents such as dichloromethane, chloroform,acetone, tetrahydrofuran, dioxane, acetonitrile, methyl isobutyl ketone,ethyl alcohol, dimethylformamide, dimethylacetamide, dimethyl sulfoxide,nitromethane, hexamethylphosphoric triamide, sulfolane, and the like;non-polar solvents such as benzene, toluene, petroleum ether, n-hexaneand the like; and a mixture thereof. These solvents can be used incombination with water.

The reactive derivatives of the compound (II) mean reactive derivativesof a carboxyl group, for example, an acid halide, an acid anhydride, anacid azolide, an active ester, an acid azide and the like. Referringmore particularly to these reactive derivatives, examples include mixedacid anhydrides or symmetric acid anhydrides with acids such as dialkylphosphoric acids, phenyl phosphoric acid, diphenyl phosphoric acid,dibenzyl phosphoric acid, halogenated phosphoric acids, dialkylphosphorous acids, methanesulfonic acid, toluenesulfonic acid,naphthalenesulfonic acid, alkylcarbonates, aliphatic carboxylic acids(for example, pivalic acid, pentanoic acid, isopentanoic acid,2-ethylbutanoic acid); acid azolides with imidazole, substitutedimidazoles, dimethylpyrazole, triazole, and the like; and active esterssuch as cyanomethyl ester, methoxymethyl ester, vinyl ester, propargylester, p-nitrophenyl ester, 2,4-dinitrophenyl ester, trichlorophenylester, pentachlorophenyl ester, methanesulfonylphenyl ester,p-nitrophenylthio ester, carboxymethylthio ester, pyridyl ester,piperidyl ester, 8-quinolylthio ester, and esters with1-hydroxy-2(1H)-pyridone, N-hydroxysuccinimide or N-hydroxyphthalimide.

Further, when the compounds of the formula (II) are used in the form ofthe free acid (or the salt thereof), it is preferred to carry out thereaction in the presence of coupling agents such asN,N'-dicyclohexylcarbodiimide,N-cyclohexyl-N-morpholinoethylcarbodiimide,N-cyclohexyl-N-(4-diethylaminocyclohexyl)-carbodiimide,N,N'-diethylcarbodiimide, N,N'-diisopropylcarbodiimide,N-ethyl-N-(3-dimethylaminopropyl)carbodiimide,N,N'-carbonyldi-(2-methylimidazole),pentamethyleneketene-N-cyclohexylimine,diphenylketene-N-cyclohexylimine, 2-ethyl-7-hydroxybenzisoxazolium salt,2-ethyl-5-(m-sulfonyl)isoxazolium hydroxide inner salt,(chloromethylene)dimethyl ammonium chloride and the like.

As described above, those amidating agents which are generally used inthe fields of peptide chemistry, penicillin chemistry and cephalosporinchemistry can be used in the present invention.

Examples of salts of compounds of formula (III) include an alkali metalsalt or an alkaline earth metal salt (for example, the sodium,potassium, calcium, etc. salts) of acids of the formula (III); organicamine salts (for example, trimethylamine, triethylamine, quinoline,collidine, etc. salts) of the acids of the formula (III); and organicsulfonic acid salts (for example, toluenesulfonic acid,naphthalenesulfonic acid, tetralinsulfonic acid, etc. salts) of theacids of the formula (III). Examples of derivatives of compounds of theformula (III) include carboxyl derivatives in which the carboxyl groupis protected by esterification or amidation, or is in the form of theanhydride thereof.

The carboxyl-protecting group can be removed after the acylationreaction under mild conditions, for example, by a solvolysis such as ahydrolysis and an alcoholysis, a catalytic hydrogenation, a reduction,an oxidation, a nucleophilic substitution reaction, a photochemicalreaction or an enzymatic reaction.

Examples of groups formed by suitable carboxyl protecting groups includea silyl ester, an organo-tin ester, a toluenesulfonyl ethyl ester, ap-nitrobenzyl ester, a benzyl ester, a phenacyl ester, a 2-furylmethylester, a diphenylmethyl ester, a substituted diphenylmethyl ester, ap-methoxybenzyl ester, a trityl ester, a benzoloxymethyl ester, a loweralkanoyl oxymethyl ester, a dimethylmethyleneamino ester, ap-nitrophenyl ester, a methylsulfonylphenyl ester, a methylthiophenylester, a t-butyl ester, a 4-picolyl ester, an iodoethyl ester, atrichloroethyl ester, a phthalimidomethyl ester, a 3,4-dimethoxy or a3,5-dimethylbenzyl ester, a 2-nitrobenzyl ester, a 2,2'-dinitrobenzylester, an acetyloxycarbonyl group, a trichloroethyl ester, a ##STR7##group, a --COON═CHR' group (in which R' is an alkyl group or an arylgroup), a ##STR8## group and the like, which are formed from carboxylprotecting groups conventionally used in the fields of peptide,penicillin and cephalosporin chemistry.

In case of the silyl ester, other substituents of the compound of theformula (III), if any, such as a hydroxy group or an amino group may besilylated.

In case of these derivatives of compounds of the formula (III), theirhydrochloric acid, p-toluenesulfonic acid, naphthalene sulfonic acid ortetralin sulfonic acid salts may also be used.

The reaction between the acid represented by the formula (II) or thereactive derivative thereof and a 7-α-amino-acylamido-cephalosporinrepresented by the formula (III) or the derivative thereof can becarried out at any temperature and usually below about 50° C.

Furthermore, the compounds of the formula (I) where X is S-Het of thepresent invention can also be prepared by reacting anN-acylamino-α-arylacetamido-cephalosporin of the formula (IV), ##STR9##wherein A and R are each as defined above, with a thiol represented bythe formula (V),

    hs--het                                                    (V)

wherein Het is as defined above.

Various well-known methods (as described in Japanese Patent PublicationNos. 12136/1971, 2340/1971, 14734/1971, Japanese Patent Application(OPI) No. 68593/1973 and Journal of the Chemical Society, 1965, 5015)can be applied in the preparation.

Further, another preparation of the compounds of the formula (I) is amethod which comprises reacting an acylamino carboxylic acid of theformula (VI), ##STR10## wherein A and R are each as defined above, or areactive derivative thereof with a compound of the formula (VII),##STR11## wherein X is as defined above, or a derivative thereof, andwhen X is an --OCOCH₃ group, further reacting the resulting reactionproduct, if necessary, with a heterocyclic thiol of the formula (V),

    hs--het                                                    (V)

in which Het is as defined above.

The compounds of the formula (III) can easily be prepared by the methodswhich are disclosed in, for example, U.S. Pat. Nos. 3,634,416 and3,634,418, Dutch Pat. No. 70/05519, Canadian Patent No. 873869, JapanesePatent Publication No. 14457/1971, and Japanese Patent Application (OPI)Nos. 49983/1974, 12579/1972, 31689/1974, 54393/1974 and 49984/1974.

It has now been found that the novel cephalasporins of the formula (I)above have a strong antimicrobial activity against gram-positive as wellas gram-negative bacteria with excellent pharmacokinetic properties andare useful as chemotherapeutic agents for infectious diseases of animalsincluding human beings, poultry and cattle. The compounds of the formula(I), for instance, display noticeable antimicrobial activity againstbacteria to which known cephalosporins series compounds are hardlyeffective such as Pseudomonas aeraginosa, indole positive Proteus,Serratia and Enterobacter aerogeneous. The cephalosporins of the formula(I), which characteristically have a phenyl group substituted with atleast one of a ureido group, an amino group, a hydroxymethyl group or ahydroxy group represented by R, are much better distributed in serum andurine than are similar compounds without such substituents.

The compound of the general formula (I) can be administeredintramuscularly or intravenously, for example, in the form of asolution, a suspension and the like.

Compositions or preparations containing the compound of the generalformula (I) as an active ingredient can be prepared by admixing thecompound of the formula (I) with one or more pharmaceutically acceptablecarriers or diluents such as water.

A usual dosage of the compound of the formula (I) is about 400 mg to 20g/day, preferably about 500 mg to 2 g day, in single or multiple doses,generally multiple doses, for an adult (about 60 Kg of body weight).

The following examples are intended to illustrate the preparation ofcompounds of the invention but are not to be construed as limiting thescope thereof. Unless otherwise indicated, all parts, percents, ratiosand the like are by weight.

EXAMPLE 1 Preparation of7-[D-α-(4-Hydroxypyridine-3-carboxyamido)-α-p-ureidophenylacetamido]cephalosporanicAcid ##STR12##

To a solution of 4.63 g of7-(D-α-amino-p-ureidophenylacetamido)cephalosporanic acid and 2.02 g oftriethylamine in 80 ml of dimethylformamide were added 2.36 g of4-hydroxypyridine-3-carboxylic acid N-hydroxysuccinimide ester understirring. After the mixture was reacted for 3 hours at room temperature,1.66 g of sodium 2-ethylhexanoate was added thereto, and after 10minutes 200 ml of dichloromethane and 100 ml of diethyl ether werefurther added thereto to deposit crystals. The crystals were collectedby filtration and washed with diethyl ether. The product obtained wasdissolved in water and the solution was adjusted to a pH of 2 underice-cooling by adding a 3N-hydrochloric acid aqueous solution theretowith stirring. The deposited crystals were collected by filtration,washed with water and dried over phosphorus pentoxide under reducedpressure. Thus 2.6 g of the titled compound was obtained.

The free acid thus obtained was dissolved in dimethylformamide and anequimolar amount of sodium 2-ethylhexanoate and then diethyl ether wereadded thereto to deposit crystals. Thus the sodium salt of the titledcompound was obtained.

In the same manner as described in Example 1, the following compoundswere synthesized. ##STR13##

EXAMPLE 10 Preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carboxyamido)-α-(p-ureidophenyl)acetamido]cephalosporanicAcid

A solution of 4.63 g of7-(D-α-amino-p-ureidophenylacetamido)cephalosporanic acid and 2.02 g oftriethylamine in 100 ml of dichloromethane was cooled with ice and 2.45g of 4-hydroxy-1,5-naphthyridine-3-carboxylic acid chloridehydrochloride were added thereto with stirring. After stirring for 20minutes, 1.01 g of triethylamine and 30 ml of dimethylformamide wereadded and the mixture was reacted for 15 hours at the same temperaturewith stirring. The insoluble matter was filtered off and the filtratewas extracted with three 30 ml portions of an aqueous sodium bicarbonatesolution. The aqueous extract was cooled with ice and adjusted to a pHof 2 under stirring with a 3N-hydrochloric acid aqueous solution. Thedeposited crystals were collected by filtration, washed with water anddried over phosphorus pentoxide under reduced pressure. Thus 2.4 g ofthe titled compound was obtained. The product was further converted tothe sodium salt thereof using a conventional method.

EXAMPLE 11 Preparation of 7-[D-α-(4-Hydrocinnoline-3-carboxyamido)-α-(p-ureidophenylacetamido)]cephalosporanic Acid ##STR14##

In 60 ml of dry dimethylformamide was dissolved 1.90 g of4-hydroxycinnoline-3-carboxylic acid at room temperature (about 20°-30°C.) with stirring. To the resulting solution was added 1.78 g ofcarbonyldiimidazole and the mixture was stirred for 30 minutes.Thereafter, 4.63 g of7-(D-α-amino-p-ureidophenylacetamido)cephalosporanic acid and 2.0 g oftriethylamine were added thereto and the mixture was reacted for 3 hoursat room temperature with stirring. To the reaction solution was added3.64 g of a 50% sodium 2-ethylhexanoate solution in n-butanol and themixture was stirred for 10 minutes. The reaction solution was pouredinto acetone and the deposited crystals were collected by filtration.The crystals were dissolved in water and the resulting solution wascooled with ice and adjusted to a pH of 2 with stirring with a2N-hydrochloric acid aqueous solution. The deposited crystals werecollected by filtration, washed with water and dried over phosphoruspentoxide under reduced pressure. Thus 3.0 g of the titled compound wasobtained. The compound was converted to the sodium salt using aconventional method.

EXAMPLE 12 Preparation of7-[D-α-(4-Hydroxypyridine-3-carboxyamido)-α-(p-ureidophenyl)acetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylic Acid ##STR15##

To a mixture of 5.35 g of7-(D-α-amino-p-ureidophenylacetamido)-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid, 2.02 g of triethylamine and 80 ml of dimethylformamide was added2.36 g of 4-hydroxypyridine-3-carboxylic acid N-hydroxysuccinimideester. After the mixture was reacted for 3 hours at room temperaturewith stirring, 1.66 g of sodium 2-ethylhexanoate was added thereto andafter 10 minutes 200 ml of acetone and 100 ml of diethyl ether werefurther added thereto to deposit crystals. The crystals were collectedby filtration, washed with diethyl ether and dissolved in water. Theresulting solution was adjusted to a pH of 2 under ice-cooling with a2N-hydrochloric acid aqueous solution. The deposited crystals werecollected by filtration, washed with water and dried over phosphoruspentoxide under reduced pressure. Thus 3.2 g of the titled compound wasobtained. The compound was further converted to the sodium salt using aconventional method.

EXAMPLE 13 Preparation of7-[D-α-(4-Hydroxypyridine-3-carboxyamido)-α-(p-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl)-thiomethyl-3-cephem-4-carboxylicAcid

In the same manner as described in Example 12, the titled compound wasobtained using7-(D-α-amino-p-ureidophenylacetamido)-3-(1-methyltetrazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid.

In the same manner, the following compounds were synthesized.

    __________________________________________________________________________     ##STR16##                                                                    Example                                                                       No.  HOA              R        Het       M                                    __________________________________________________________________________    14                                                                                  ##STR17##                                                                                      ##STR18##                                                                              ##STR19##                                                                              Na                                   15                                                                                  ##STR20##                                                                                      ##STR21##                                                                              ##STR22##                                                                              Na                                   16                                                                                  ##STR23##                                                                                      ##STR24##                                                                              ##STR25##                                                                              Na                                   17                                                                                  ##STR26##                                                                                      ##STR27##                                                                              ##STR28##                                                                              Na                                   18                                                                                  ##STR29##                                                                                      ##STR30##                                                                              ##STR31##                                                                              Na                                   19                                                                                  ##STR32##                                                                                      ##STR33##                                                                              ##STR34##                                                                              Na                                   20                                                                                  ##STR35##                                                                                      ##STR36##                                                                              ##STR37##                                                                              Na                                   21                                                                                  ##STR38##                                                                                      ##STR39##                                                                              ##STR40##                                                                              Na                                   22                                                                                  ##STR41##                                                                                      ##STR42##                                                                              ##STR43##                                                                              Na                                   23                                                                                  ##STR44##                                                                                      ##STR45##                                                                              ##STR46##                                                                              Na                                   24                                                                                  ##STR47##                                                                                      ##STR48##                                                                              ##STR49##                                                                              Na                                   25                                                                                  ##STR50##                                                                                      ##STR51##                                                                              ##STR52##                                                                              Na                                   26                                                                                  ##STR53##                                                                                      ##STR54##                                                                              ##STR55##                                                                              Na                                   27                                                                                  ##STR56##                                                                                      ##STR57##                                                                              ##STR58##                                                                              Na                                   28                                                                                  ##STR59##                                                                                      ##STR60##                                                                              ##STR61##                                                                              Na                                   29                                                                                  ##STR62##                                                                                      ##STR63##                                                                              ##STR64##                                                                              Na                                   __________________________________________________________________________

EXAMPLE 30 Preparation of7-[D-α-(4-Hydroxypyridine-3-carboxyamido)-α-(p-ureidophenyl)acetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid ##STR65##

A mixture of 1.21 g of sodium7-[D-α-(4-hydroxypyridine-3-carboxyamido)-α-(p-ureidophenyl)acetamido]cephalosporanate,0.20 g of sodium bicarbonate, 0.36 g of2-methyl-5-mercapto-1,3,4-thiadiazole and 25 ml of a phosphate buffer(0.1N KH₂ PO₄ -0.1N NaHPO₄ ; 2:1 by volume; pH:6.3) was stirred for 6hours at 60° C. Then the solution was cooled with ice and made acidic toa pH of 2 with a 3N-hydrochloric acid aqueous solution. The depositedcrystals were collected by filtration, washed with water and dried overphosphorus pentoxide under reduced pressure. Thus 0.62 g of the titledcompound was obtained. The compound was further converted into thesodium salt using a conventional method.

The compounds obtained in Examples 15, 16, 20, 22 and 26 were alsoobtained in the same manner as described in Example 30.

The following compounds were obtained in the same manner as described inExamples 1 to 12.

    __________________________________________________________________________                 ##STR66##                                                    

    __________________________________________________________________________     No.Example  HOA                                                                                              ##STR67##                                     __________________________________________________________________________    31                                                                                         ##STR68##                                                                                        ##STR69##                                     32                                                                                         ##STR70##                                                                                        ##STR71##                                     33                                                                                         ##STR72##                                                                                        ##STR73##                                     34                                                                                         ##STR74##                                                                                        ##STR75##                                     35                                                                                         ##STR76##                                                                                        ##STR77##                                      ##STR78##                                                                    __________________________________________________________________________     No.Example                                                                         HOA                                                                                           ##STR79##    Het                                        __________________________________________________________________________    36                                                                                  ##STR80##                                                                                     ##STR81##                                                                                  ##STR82##                                  37                                                                                  ##STR83##                                                                                     ##STR84##                                                                                  ##STR85##                                  38                                                                                  ##STR86##                                                                                     ##STR87##                                                                                  ##STR88##                                  39                                                                                  ##STR89##                                                                                     ##STR90##                                                                                  ##STR91##                                  40                                                                                  ##STR92##                                                                                     ##STR93##                                                                                  ##STR94##                                  41                                                                                  ##STR95##                                                                                     ##STR96##                                                                                  ##STR97##                                  42                                                                                  ##STR98##                                                                                     ##STR99##                                                                                  ##STR100##                                 43                                                                                  ##STR101##                                                                                    ##STR102##                                                                                 ##STR103##                                 44                                                                                  ##STR104##                                                                                    ##STR105##                                                                                 ##STR106##                                 45                                                                                  ##STR107##                                                                                    ##STR108##                                                                                 ##STR109##                                 46                                                                                  ##STR110##                                                                                    ##STR111##                                                                                 ##STR112##                                 47                                                                                  ##STR113##                                                                                    ##STR114##                                                                                 ##STR115##                                 48                                                                                  ##STR116##                                                                                    ##STR117##                                                                                 ##STR118##                                 49                                                                                  ##STR119##                                                                                    ##STR120##                                                                                 ##STR121##                                 __________________________________________________________________________

example 50 preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carboxyamido)-α-p-aminophenylacetamido]cephalosporanicAcid ##STR122##

To a mixture of 4.20 g of 7-(D-α-p-aminophenylacetamido)cephalosporanicacid, 2.02 g of triethylamine and 50 ml of dimethylformamide were added2.87 g of 4-hydroxy-1,5-naphthyridine-3-carboxylic acidN-hydroxysuccinimide ester, and the mixture was stirred for 2 hours atroom temperature. The insoluble matter was filtered off and to thefiltrate were added 100 ml of dichloromethane and 300 ml of diethylether. The deposited crystals were collected by filtration, washed withdiethyl ether and dried over phosphorus pentoxide under reducedpressure. Thus 4.7 g of the triethylamine salt of the titled compoundwas obtained.

The compound was dissolved in dimethylformamide and to the resultingsolution was added sodium 2-ethylhexanoate in an amount of 1.1 molartimes the titled compound. On further adding acetone to the solution,the sodium salt of the titled compound separated as crystals.

EXAMPLE 51 Preparation of 7-[D-α-(4-Hydroxypyridine-3-carbonamide)-α-m-aminophenylacetamido]cephalosporanic acid ##STR123##

To a mixture of 4.20 g of 7-[D-α-m-aminophenylacetamido]-cephalosporanicacid, 2.02 g of triethylamine and 50 ml of dimethylformamide was added2.36 g of 4-hydroxypyridine-3-carboxylic acid N-hydroxysuccinimideester, and the mixture was stirred for 2 hours at room temperature.

Then 1.66 g of sodium 2-ethylhexanoate was added and, after 10 minutes,100 ml of dichloromethane and 200 ml of diethyl ether were further addedto deposit crystals. The crystals were collected by filtration, washedwith diethyl ether and dried over phosphorus pentoxide under reducedpressure. Thus 4.5 g of the sodium salt of the titled compound wasobtained.

EXAMPLE 52 Preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-aminophenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5yl)thiomethyl-3-cephem-4-carboxylicacid ##STR124##

The titled compound was obtained in the same manner as described inExample 1 except that 7-(D-α-p-aminophenylacetamido)-3-(2-methyl-1,3,4-thiadiazol-5yl)thiomethyl-3-cephem-4-carboxylicacid was used in place of 7-(D-α-p-aminophenylacetamido)cephalosporanicacid.

In the same manner as described in Examples 50 to 52, the followingcompounds were synthesized.

    __________________________________________________________________________     ##STR125##                                                                   Example                                                                       No.  HOA            R        X           M                                    __________________________________________________________________________    53                                                                                  ##STR126##                                                                                   ##STR127##                                                                            OCOCH.sub.3 Na                                   54                                                                                  ##STR128##                                                                                   ##STR129##                                                                             ##STR130## Na                                   55                                                                                  ##STR131##                                                                                   ##STR132##                                                                             ##STR133## Na                                   56                                                                                  ##STR134##                                                                                   ##STR135##                                                                             ##STR136## Na                                   57                                                                                  ##STR137##                                                                                   ##STR138##                                                                             ##STR139## Na                                   58                                                                                  ##STR140##                                                                                   ##STR141##                                                                            OCOCH.sub.3 Na                                   59                                                                                  ##STR142##                                                                                   ##STR143##                                                                             ##STR144## Na                                   60                                                                                  ##STR145##                                                                                   ##STR146##                                                                             ##STR147## Na                                   61                                                                                  ##STR148##                                                                                   ##STR149##                                                                             ##STR150## Na                                   62                                                                                  ##STR151##                                                                                   ##STR152##                                                                            OCOCH.sub.3 Na                                   63                                                                                  ##STR153##                                                                                   ##STR154##                                                                             ##STR155## Na                                   __________________________________________________________________________

EXAMPLE 64 Preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-m-aminophenylacetamido]-3-(1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid ##STR156##

A mixture of 1.18 g of7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carbonamido)-α-m-aminophenylacetamido]-cephalosporanicacid, 0.368 g of sodium bicarbonate, 0.32 g of2-mercapto-1,3,4-thiadiazole and 25 ml of a phosphate buffer (0.1N KH₂PO₄ -0.1N NaHPO₄ ; 2:1 by volume; pH:6.3) was stirred for 5.5 hours at60° C. Then 20 ml of ethanol was added and the mixture was kept at 0° to5° C. overnight. The deposited crystals were collected by filtration,washed with ethanol and dried over silica gel under reduced pressure.Thus, 1.05 g of the sodium salt of the titled compound was obtained.

The following compounds were obtained in the same manner as described inExamples 50 to 52 and 64.

    __________________________________________________________________________     ##STR157##                                                                    Example No.                                                                          HOA                                                                                            ##STR158##                                                                             X                                           __________________________________________________________________________    65                                                                                    ##STR159##                                                                                     ##STR160##                                                                             ##STR161##                                  66                                                                                    ##STR162##                                                                                     ##STR163##                                                                             ##STR164##                                  67                                                                                    ##STR165##                                                                                     ##STR166##                                                                             ##STR167##                                  68                                                                                    ##STR168##                                                                                     ##STR169##                                                                             ##STR170##                                  69                                                                                    ##STR171##                                                                                     ##STR172##                                                                             ##STR173##                                  70                                                                                    ##STR174##                                                                                     ##STR175##                                                                             ##STR176##                                  71                                                                                    ##STR177##                                                                                     ##STR178##                                                                             ##STR179##                                  72                                                                                    ##STR180##                                                                                     ##STR181##                                                                             ##STR182##                                  73                                                                                    ##STR183##                                                                                     ##STR184##                                                                             ##STR185##                                  74                                                                                    ##STR186##                                                                                     ##STR187##                                                                             ##STR188##                                  75                                                                                    ##STR189##                                                                                     ##STR190##                                                                             ##STR191##                                  76                                                                                    ##STR192##                                                                                     ##STR193##                                                                             ##STR194##                                  77                                                                                    ##STR195##                                                                                     ##STR196##                                                                             ##STR197##                                  78                                                                                    ##STR198##                                                                                     ##STR199##                                                                             ##STR200##                                  79                                                                                    ##STR201##                                                                                     ##STR202##                                                                             ##STR203##                                  80                                                                                    ##STR204##                                                                                     ##STR205##                                                                             ##STR206##                                  81                                                                                    ##STR207##                                                                                     ##STR208##                                                                             ##STR209##                                  82                                                                                    ##STR210##                                                                                     ##STR211##                                                                             ##STR212##                                  83                                                                                    ##STR213##                                                                                     ##STR214##                                                                             ##STR215##                                  84                                                                                    ##STR216##                                                                                     ##STR217##                                                                             ##STR218##                                  85                                                                                    ##STR219##                                                                                     ##STR220##                                                                             ##STR221##                                  86                                                                                    ##STR222##                                                                                     ##STR223##                                                                             ##STR224##                                  87                                                                                    ##STR225##                                                                                     ##STR226##                                                                             ##STR227##                                  __________________________________________________________________________

example 88 preparation of7-[DL-α-(4-Hydroxypyridine-3-carbonamido)-α-p-hydroxymethylphenylacetamido]cephalosporanicAcid ##STR228##

To a solution of 4.35 g of7-[DL-α-p-hydroxymethylphenylacetamido]cephalosporanic acid and 2.02 gof triethylamine in 80ml of dichloromethane were added under ice-cooling2.36 g of 4-hydroxypyridine-3-carboxylic acid N-hydroxysuccinimide esterand 40 ml of dimethylformamide with stirring, and the mixture wasstirred for 30 minutes at the same temperature. After the mixture wasfurther reacted for 2 hours at room temperature with stirring, 1.66 g ofsodium 2-ethylhexanoate was added, and, after 10 minutes, 100 ml ofdichloromethane and 200 ml of diethyl ether were further added theretoto deposit crystals. The crystals were collected by filtration, washedwith diethyl ether and dried over phosphorus pentoxide under reducedpressure. Thus 4.0 g of the sodium salt of the titled compound wasobtained.

EXAMPLE 89 Preparation of7-[DL-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxymethylphenylacetamido]cephalosporanicAcid ##STR229##

To a solution of 4.35 g of7-[DL-α-p-hydroxymethylphenylacetamido]cephalosporanic acid and 2.02 gof triethylamine in 60 ml of dimethylformamide were added 2.87 g of4-hydroxy-1,5-naphthyridine-3-carboxylic acid N-hydroxysuccinimideester, and the mixture was stirred for 2 hours at room temperature. Theinsoluble matter was filtered off and 200 ml of dichloromethane and 350ml of diethyl ether were added to the filtrate to deposit crystals. Thecrystals were collected by filtration, washed with diethyl ether anddried over phosphorus pentoxide under reduced pressure. Thus 4.2 g ofthe triethylamine salt of the tilted compound was obtained.

The compound was converted to the sodium salt using a conventionalmethod.

EXAMPLE 90 Preparation of 7-[DL-α-(4-Hydroxypyridine-3carbonamido)-α-p-hydroxymethylphenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicAcid ##STR230##

To a solution of 5.07 g of7-(DL-α-p-hydroxymethylphenylacetamido)-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid and 2.02 g of triethylamine in 80 ml of dimethylformamide wereadded 2.36 g of 4-hydroxypyridine-3-carboxylic acid N-hydroxysuccinimideester with stirring, and the mixture was reacted for 2 hours at roomtemperature with stirring. Then 1.66 g of sodium 2-ethylhexanoate wasadded and, after 10 minutes, 200 ml of acetone and 100 ml of diethylether were further added thereto to deposit crystals. The crystals werecollected by filtration, washed with diethyl ether and dried overphosphorus pentoxide under reduced pressure. Thus 4.8 g of the sodiumsalt of the titled compound was obtained.

In the same manner as described in Examples 88 to 90, the followingcompounds were synthesized.

    __________________________________________________________________________     ##STR231##                                                                   Example                                                                       No.    HOA             R       X             M                                __________________________________________________________________________    91                                                                                    ##STR232##                                                                                    ##STR233##                                                                            ##STR234##   Na                               92                                                                                    ##STR235##                                                                                    ##STR236##                                                                           OCOCH.sub.3   Na                               93                                                                                    ##STR237##                                                                                    ##STR238##                                                                            ##STR239##   Na                               94                                                                                    ##STR240##                                                                                    ##STR241##                                                                            ##STR242##   Na                               95                                                                                    ##STR243##                                                                                    ##STR244##                                                                            ##STR245##   Na                               96                                                                                    ##STR246##                                                                                    ##STR247##                                                                            ##STR248##   Na                               97                                                                                    ##STR249##                                                                                    ##STR250##                                                                            ##STR251##   Na                               98                                                                                    ##STR252##                                                                                    ##STR253##                                                                            ##STR254##   Na                               99                                                                                    ##STR255##                                                                                    ##STR256##                                                                           OCOCH.sub.3   Na                               100                                                                                   ##STR257##                                                                                    ##STR258##                                                                            ##STR259##   Na                               __________________________________________________________________________

EXAMPLE 101 Preparation of7-[DL-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxymethylphenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylic Acid ##STR260##

A mixture of 3.03 g of7-[DL-α-(4-hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxymethylphenylacetamido]cephalosporanicacid, 0.92 g of sodium bicarbonate, 0.90 g of2-methyl-5-mercapto-1,3,4-thiadiazole and 60 ml of a phosphate buffer(0.1 N KH₂ PO₄ -- 0.1 N NaHPO₄ ; 2:1 by volume; pH 6.3) was stirred for5.5 hours at 50° to 60° C. Then 40 ml of ethanol was added thereto andthe mixture was kept at 0° to 5° C. overnight. The deposited crystalswere collected by filtration, washed with ethanol and dried overphosphorus pentoxide under reduced pressure. Thus 2.5 g of the sodiumsalt of the titled compound was obtained.

The compounds obtained in Examples 91, 94 and 98 were obtained in thesame manner as described in Example 101.

The following compounds were obtained in the same manner as described inExamples 88 to 90.

    __________________________________________________________________________     ##STR261##                                                                   Example                                                                       No.   HOA               R       X             M                               __________________________________________________________________________    102                                                                                  ##STR262##                                                                                      ##STR263##                                                                            ##STR264##   Na                              103                                                                                  ##STR265##                                                                                      ##STR266##                                                                            ##STR267##   Na                              104                                                                                  ##STR268##                                                                                      ##STR269##                                                                            ##STR270##   Na                              105                                                                                  ##STR271##                                                                                      ##STR272##                                                                            ##STR273##   Na                              106                                                                                  ##STR274##                                                                                      ##STR275##                                                                            ##STR276##   Na                              107                                                                                  ##STR277##                                                                                      ##STR278##                                                                            ##STR279##   Na                              108                                                                                  ##STR280##                                                                                      ##STR281##                                                                            ##STR282##   Na                              109                                                                                  ##STR283##                                                                                      ##STR284##                                                                            ##STR285##   Na                              110                                                                                  ##STR286##                                                                                      ##STR287##                                                                            ##STR288##   Na                              111                                                                                  ##STR289##                                                                                      ##STR290##                                                                            ##STR291##   Na                              112                                                                                  ##STR292##                                                                                      ##STR293##                                                                            ##STR294##   Na                              __________________________________________________________________________

EXAMPLE 113 Preparation of7-[D-α-(4-Hydroxypyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]cephalosporanicAcid ##STR295##

To a mixture of 4.21 g of 7[D-α-p-hydroxyphenylacetamido]cephalosporanicacid, 60 ml of dichloromethane and 2.02 g of triethylamine were addedunder ice-cooling 2.36 g of 4-hydroxypyridine-3-carboxylic acidN-hydroxysuccinimide ester and 30 ml of dimethylformamide with stirring,and the mixture was stirred for 30 minutes at the same temperature.After the mixture was further reacted for 2 hours at room temperaturewith stirring, 1.66 g of sodium 2-ethylhexanoate was added and, after 10minutes, 200 ml of dichloromethane and 100 ml of diethyl ether werefurther added thereto to deposit crystals. The crystals were collectedby filtration, and washed with diethyl ether. The crystals weredissolved in water and the resulting solution was cooled with ice andmade acidic to a pH of 2 under stirring with a 3N-hydrochloric acidaqueous solution. The deposited crystals were collected by filtration,washed with water and dried over phosphorus pentoxide under reducedpressure. Thus 2.1 g of the titled compound was obtained. The free acidthus obtained was added to 13 ml of dimethylformamide and 1.1 molarequivalents of sodium 2-ethylhexanoate was added thereto. On furtheradding dichloromethane and acetone to deposit crystals, 1.9 g of thesodium salt of the titled compound was obtained as crystals.

The following compounds were obtained in the same manner as described inExample 113.

    ______________________________________                                         ##STR296##                                                                   Example No.                                                                            HOA             R                                                    ______________________________________                                        114                                                                                     ##STR297##                                                                                    ##STR298##                                          115                                                                                     ##STR299##                                                                                    ##STR300##                                          116                                                                                     ##STR301##                                                                                    ##STR302##                                          117                                                                                     ##STR303##                                                                                    ##STR304##                                          118                                                                                     ##STR305##                                                                                    ##STR306##                                          119                                                                                     ##STR307##                                                                                    ##STR308##                                          ______________________________________                                    

example 120 preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-hydroxyphenylacetamido]cephalosporanicAcid ##STR309##

To a solution of 4.21 g of7-[D-α-p-hydroxyphenylacetamido]cephalosporanic acid and 1.975 g oftriethylamine in 80 ml of dimethylformamide were added 2.87 g of4-hydroxy-1,5-naphthyridine-3-carboxylic acid N-hydroxysuccinimideester, and the mixture was stirred for 2 hours at room temperature. Theinsoluble matter was filtered off and to the filtrate were added 80 mlof dichloromethane and 350 ml of diethyl ether. The deposited crystalswere collected by filtration, washed with diethyl ether and dried overphosphorus pentoxide under reduced pressure. Thus 4.4 g of thetriethylamine salt of the titled compound was obtained. The compound wasconverted to the sodium salt using a conventional method.

EXAMPLE 121 Preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]cephalosporanicAcid

To an ice-cooled solution of 4.21 g of7-[D-α-p-hydroxyphenylacetamido]cephalosporanic acid and 2.02 g oftriethylamine in 100 ml of dichloromethane was added 2.45 g of4-hydroxy-1,5naphthyridine-3-carboxylic acid chloride hydrochloride withstirring, and the mixture was stirred for 20 minutes. Thereafter 1.01 gof triethylamine was added and the mixture was reacted for 3 hours atthe same temperature. The insoluble matter was filtered off and thefiltrate was extracted with three 20 ml portions of an aqueous sodiumbicarbonate solution. The aqueous extract was cooled with ice and madeacidic to a pH of 2 with a 1N-hydrochloric acid aqueous solution understirring. The deposited crystals were collected by filtration, washedwith water, methanol, and then diethyl ether and dried over phosphoruspentoxide under reduced pressure. Thus 2.2 g of the titled compound wasobtained. The compound was converted to the sodium salt using sodium2-ethylhexanoate.

The following compounds were synthesized in the same manner as describedin Example 120.

    ______________________________________                                         ##STR310##                                                                   Example No.                                                                            HOA               R           M                                      ______________________________________                                        122                                                                                     ##STR311##                                                                                      ##STR312## Na                                     123                                                                                     ##STR313##                                                                                      ##STR314## Na                                     ______________________________________                                    

EXAMPLE 124 Preparation of7-[D-α-(4-hydroxycinnoline-3-carbonamido)-α-p-hydrxyphenylacetamido]cephalosporanicacid ##STR315##

In 60 ml of dry dimethylformamide was dissolved 1.90 g of4-hydroxycinnoline-3-carboxylic acid at room temperature with stirring,and 1.78 g of carbonyldiimidazole was added thereto followed by stirringfor 30 minutes. Then 4.21 g of7-[D-α-p-hydroxyphenylacetamido]cephalosporanic acid and 1.8 g oftriethylamine were added thereto and the mixture was stirred for 3 hoursat room temperature. Thereafter 3.64 g of a 50% sodium 2-ethylhexanoatesolution in n-butanol were added followed by stirring for 10 minutes.The reaction solution was poured into acetone and the deposited crystalswere collected by filtration. The crystals were dissolved in water andthe resulting solution was cooled with ice and adjusted to a pH of 2with a 1N-hydrochloric acid aqueous solution with stirring. Thedeposited crystals were collected by filtration and dried overphosphorus pentoxide under reduced pressure. Thus 3.2 g of the titledcompound was obtained. The compound was converted to the sodium saltusing sodium 2-ethylhexanoate.

EXAMPLE 125 Preparation of7-[D-α-(4-Hydroxypyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylic Acid. ##STR316##

To a mixture of 4.93 g of7-(D-α-p-hydroxyphenylacetamido)-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid, 2.02 g of triethylamine and 80 ml of dimethylformamide was addedunder ice-cooling 2.36 g of 4-hydroxypyridine-3-carboxylic acidN-hydroxysuccinimide ester with stirring, and the mixture was stirredfor 30 minutes at the same temperature. After the mixture was furtherreacted for 2 hours at room temperature with stirring, 1.66 g of sodium2-ethylhexanoate was added and, after 10 minutes, 200 ml of acetone and100 ml of diethyl ether were further added thereto to deposit crystals.The crystals were collected by filtration and washed with diethyl ether.The crystals were dissolved in water and the resulting solution wascooled with ice and made acidic to a pH of 2 with a 2N-hydrochloric acidaqueous solution. The deposited crystals were collected by filtration,washed with water and dried over phosphorus pentoxide under reducedpressure. Thus 2.6 g of the titled compound was obtained.

A mixture of 4.93 g of7-(D-α-p-hydroxyphenylacetamido)-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid, 2.02 g of triethylamine and 80 ml of dimethyl sulfoxide wasstirred at room temperature and 2.36 g of 4-hydroxypyridine-3-carboxylicacid N-hydroxysuccinimide ester were added thereto. After the mixturewas stirred for 30 minutes, 1.66 g of sodium 2-ethylhexanoate was addedto the reaction solution and, after 10 minutes, 2.0 liters of acetonewere further added thereto to deposit crystals. The crystals werecollected by filtration, washed with acetone and diethyl ether and driedover phosphorus pentoxide under reduced pressure. Thus 5.2 g of thesodium salt of the titled compound was obtained.

EXAMPLE 126 Preparation of7-[D-α-(4-Hydroxypyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]-3-(1-methyltetrazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid

In the same manner as described in Example 125, the titled compound wasobtained using7-(D-α-p-hydroxyphenylacetamido)-3-(1-methyltetrazol-5-yl)thiomethyl-3-cephem-4-carboxylicacid.

In the same manner, the following compounds were obtained.

    ______________________________________                                         ##STR317##                                                                   Ex.                                                                           No.  HOA             R        Het        M                                    ______________________________________                                        127                                                                                 ##STR318##                                                                                    ##STR319##                                                                             ##STR320##                                                                              Na                                   128                                                                                 ##STR321##                                                                                    ##STR322##                                                                             ##STR323##                                                                              Na                                   129                                                                                 ##STR324##                                                                                    ##STR325##                                                                             ##STR326##                                                                              Na                                   130                                                                                 ##STR327##                                                                                    ##STR328##                                                                             ##STR329##                                                                              Na                                   131                                                                                 ##STR330##                                                                                    ##STR331##                                                                             ##STR332##                                                                              Na                                   132                                                                                 ##STR333##                                                                                    ##STR334##                                                                             ##STR335##                                                                              Na                                   133                                                                                 ##STR336##                                                                                    ##STR337##                                                                             ##STR338##                                                                              Na                                   134                                                                                 ##STR339##                                                                                    ##STR340##                                                                             ##STR341##                                                                              Na                                   ______________________________________                                    

EXAMPLE 135 Preparation of7-[D-α-(4-Hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylicAcid ##STR342##

A mixture of 1.18 g of7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carbonamido)-α-p-hydroxyphenylacetamido]cephalosporanicacid, 0.368 g of sodium bicarbonate, 0.36 g of2-methyl-5-mercapto-1,3,4-thiadiazole and 23 ml of a phosphate buffer(0.1 N KH₂ PO₄ -0.1 N NaHPO₄ ; 2:1 by volume; pH 6.3) was stirred for5.5 hours at 50° C. to 60° C. Then 20 ml of ethanol were added theretoand the mixture was kept at 0° to 5° C. overnight. The depositedcrystals were collected by filtration, washed with ethanol and driedover phosphorus pentoxide under reduced pressure. Thus 1.0 of the sodiumsalt of the titled compound was obtained.

The compounds obtained in Examples 125, 127 and 128 were obtained in thesame manner as described in Example 135.

The following compounds were synthesized in the same manner as describedin Example 120.

    ______________________________________                                         ##STR343##                                                                   Ex.                                                                           No.  HOA                R              M                                      ______________________________________                                        136                                                                                 ##STR344##                                                                                       ##STR345##    Na                                     137                                                                                 ##STR346##                                                                                       ##STR347##    Na                                     138                                                                                 ##STR348##                                                                                       ##STR349##    Na                                     139                                                                                 ##STR350##                                                                                       ##STR351##    Na                                     140                                                                                 ##STR352##                                                                                       ##STR353##    Na                                     141                                                                                 ##STR354##                                                                                       ##STR355##    Na                                     142                                                                                 ##STR356##                                                                                       ##STR357##    Na                                     143                                                                                 ##STR358##                                                                                       ##STR359##    Na                                     144                                                                                 ##STR360##                                                                                       ##STR361##    Na                                     145                                                                                 ##STR362##                                                                                       ##STR363##    Na                                     ______________________________________                                    

The following compounds were synthesized in the same manner as describedin Example 125.

    __________________________________________________________________________     ##STR364##                                                                   Example                                                                       No.   HOA                   R                                                 Het   M                                                                       __________________________________________________________________________    146                                                                                  ##STR365##                                                                                          ##STR366##                                                                                 ##STR367##       Na                 147                                                                                  ##STR368##                                                                                          ##STR369##                                                                                 ##STR370##       Na                 148                                                                                  ##STR371##                                                                                          ##STR372##                                                                                 ##STR373##       Na                 149                                                                                  ##STR374##                                                                                          ##STR375##                                                                                 ##STR376##       Na                 150                                                                                  ##STR377##                                                                                          ##STR378##                                                                                 ##STR379##       Na                 151                                                                                  ##STR380##                                                                                          ##STR381##                                                                                 ##STR382##       Na                 152                                                                                  ##STR383##                                                                                          ##STR384##                                                                                 ##STR385##       Na                 153                                                                                  ##STR386##                                                                                          ##STR387##                                                                                 ##STR388##       Na                 154                                                                                  ##STR389##                                                                                          ##STR390##                                                                                 ##STR391##       Na                 155                                                                                  ##STR392##                                                                                          ##STR393##                                                                                 ##STR394##       Na                 156                                                                                  ##STR395##                                                                                          ##STR396##                                                                                 ##STR397##       Na                 157                                                                                  ##STR398##                                                                                          ##STR399##                                                                                 ##STR400##       Na                 158                                                                                  ##STR401##                                                                                          ##STR402##                                                                                 ##STR403##       Na                 159                                                                                  ##STR404##                                                                                          ##STR405##                                                                                 ##STR406##       Na                 160                                                                                  ##STR407##                                                                                          ##STR408##                                                                                 ##STR409##       Na                 161                                                                                  ##STR410##                                                                                          ##STR411##                                                                                 ##STR412##       Na                 162                                                                                  ##STR413##                                                                                          ##STR414##                                                                                 ##STR415##       Na                 163                                                                                  ##STR416##                                                                                          ##STR417##                                                                                 ##STR418##       Na                 164                                                                                  ##STR419##                                                                                          ##STR420##                                                                                 ##STR421##       Na                 165                                                                                  ##STR422##                                                                                          ##STR423##                                                                                 ##STR424##       Na                 166                                                                                  ##STR425##                                                                                          ##STR426##                                                                                 ##STR427##       Na                 167                                                                                  ##STR428##                                                                                          ##STR429##                                                                                 ##STR430##       Na                 168                                                                                  ##STR431##                                                                                          ##STR432##                                                                                 ##STR433##       Na                 169                                                                                  ##STR434##                                                                                          ##STR435##                                                                                 ##STR436##       Na                 170                                                                                  ##STR437##                                                                                          ##STR438##                                                                                 ##STR439##       Na                 171                                                                                  ##STR440##                                                                                          ##STR441##                                                                                 ##STR442##       Na                 172                                                                                  ##STR443##                                                                                          ##STR444##                                                                                 ##STR445##       Na                 173                                                                                  ##STR446##                                                                                          ##STR447##                                                                                 ##STR448##       Na                 174                                                                                  ##STR449##                                                                                          ##STR450##                                                                                 ##STR451##       Na                 175                                                                                  ##STR452##                                                                                          ##STR453##                                                                                 ##STR454##       Na                 176                                                                                  ##STR455##                                                                                          ##STR456##                                                                                 ##STR457##       Na                 177                                                                                  ##STR458##                                                                                          ##STR459##                                                                                 ##STR460##       Na                 178                                                                                  ##STR461##                                                                                          ##STR462##                                                                                 ##STR463##       Na                 179                                                                                  ##STR464##                                                                                          ##STR465##                                                                                 ##STR466##       Na                 180                                                                                  ##STR467##                                                                                          ##STR468##                                                                                 ##STR469##       Na                 181                                                                                  ##STR470##                                                                                          ##STR471##                                                                                 ##STR472##       Na                  ##STR473##                                                Na                 Example                                                                       No.   HOA                    R              X                                 __________________________________________________________________________    182                                                                                  ##STR474##                                                                                           ##STR475##    OCOCH.sub.3                       183                                                                                  ##STR476##                                                                                           ##STR477##                                                                                   ##STR478##                       184                                                                                  ##STR479##                                                                                           ##STR480##                                                                                   ##STR481##                       185                                                                                  ##STR482##                                                                                           ##STR483##                                                                                   ##STR484##                       186                                                                                  ##STR485##                                                                                           ##STR486##                                                                                   ##STR487##                       187                                                                                  ##STR488##                                                                                           ##STR489##                                                                                   ##STR490##                       __________________________________________________________________________

anti-microbial activity tests were carried out on the compounds obtainedin the Examples. The results obtained are summarized in the followingTable.

    __________________________________________________________________________           Minimum Inhibitory Concentration (μg/ml)                                   Staphy-                                                                            Staphy-            Proteus                                                                             Pseudo-       Entero-                           lococcus                                                                           lococcus                                                                            Escheri-                                                                            Klebsiella                                                                           vul-  monas aeru-                                                                          Ser-   bacter ae-                 Example                                                                              aureus                                                                             aureus*                                                                             chia coli                                                                           pneumoniae                                                                           garis ginosa ratia  rogeneus                   No.    209 P                                                                              FS 289                                                                              NIHJ  602    HX 19 104    No. 72 No. 75                     __________________________________________________________________________    1      0.39 3.13  12.5  12.5   0.2   6.25   >200   25                         2      0.39 6.25  12.5  12.5   0.39  6.25   >200   25                         3      0.39 6.25  25    25     0.39  12.5   >200   25                         4      0.39 6.25  6.25  6.25   0.2   25     100    50                         5      0.39 6.25  12.5  12.5   0.39  12.5   200    50                         6      0.39 6.25  12.5  12.5   0.2   12.5   200    25                         7      0.78 6.25  3.13  3.13   0.78  6.25   50     6.25                       8      0.78 6.25  6.25  12.5   0.39  6.25   50     12.5                       9      0.78 6.25  12.5  12.5   0.39  12.5   50     12.5                       12     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       13     0.39 6.25  6.25  3.13   0.2   6.25   50     12.5                       14     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       15     0.39 6.25  12.5  3.13   0.1   6.25   50     6.25                       16     0.39 6.25  6.25  3.13   0.2   6.25   50     6.25                       17     0.39 6.25  6.25  3.13   0.1   6.25   50     12.5                       18     0.39 6.25  6.25  6.25   0.2   6.25   50     12.5                       19     0.39 6.25  12.5  6.25   0.2   12.5   50     25                         20     0.78 3.13  3.13  1.56   0.1   3.13   12.5   3.13                       21     0.78 3.13  3.13  1.56   0.2   3.13   12.5   3.13                       22     0.78 6.25  1.56  1.56   0.1   3.13   12.5   3.13                       23     1.56 6.25  3.13  1.56   0.2   3.13   12.5   3.13                       24     1.56 6.25  3.13  1.56   0.39  3.13   12.5   3.13                       25     1.56 6.25  3.13  3.13   0.39  3.13   12.5   6.25                       26     1.56 6.25  3.13  3.13   0.2   3.13   12.5   6.25                       27     1.56 6.25  3.13  1.56   0.2   3.13   12.5   3.13                       28     1.56 6.25  1.56  1.56   0.1   3.13   12.5   3.13                       29     0.78 6.25  1.56  1.56   0.2   3.13   12.5   3.13                       31     0.78 6.25  3.13  3.13   0.39  6.25   50     25                         32     0.78 6.25  3.13  3.13   0.39  6.25   50     12.5                       33     0.78 6.78  3.13  3.13   0.78  6.25   50     12.5                       34     0.78 6.25  3.13  3.13   0.78  6.25   50     12.5                       35     1.56 6.25  6.25  3.13   0.39  6.25   50     25                         36     0.78 6.25  3.13  1.56   0.2   3.13   12.5   6.25                       37     0.78 6.25  1.56  1.56   0.2   3.13   12.5   6.25                       38     0.78 6.25  1.56  1.56   0.1   3.13   12.5   3.13                       39     0.78 6.25  1.56  1.56   0.1   3.13   12.5   3.13                       40     0.78 6.25  3.13  1.56   0.1   3.13   12.5   6.25                       41     1.56 6.25  3.13  3.13   0.2   6.25   12.5   6.25                       42     1.56 6.25  3.13  3.13   0.2   6.25   12.5   6.25                       43     0.78 6.25  6.25  3.13   0.39  6.25   12.5   6.25                       44     0.78 6.25  6.25  3.13   0.39  6.25   12.5   6.25                       45     0.39 6.25  12.5  3.13   0.39  12.5   50     12.5                       46     0.78 6.25  12.5  3.13   0.39  12.5   50     12.5                       47     0.78 6.25  12.5  3.13   0.39  12.5   50     12.5                       48     0.78 12.5  12.5  3.13   0.78  25     100    25                         49     0.78 12.5  12.5  6.25   0.78  25     50     25                         50     1.56 6.25  3.13  3.13   0.39  6.25   50     6.25                       51     0.39 3.13  12.5  12.5   0.2   6.25   >200   25                         52     1.56 6.25  1.56  3.13   0.1   6.25   12.5   3.13                       53     1.56 6.25  3.13  1.56   0.39  6.25   50     6.25                       54     0.78 6.25  1.56  3.13   0.1   3.13   12.5   3.13                       55     0.78 3.13  1.56  3.13   0.1   3.13   12.5   3.13                       56     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       57     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       58     0.39 3.13  12.5  12.5   0.2   6.25   > 200  25                         59     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       60     0.39 6.25  6.25  6.25   0.1   6.25   50     12.5                       61     0.39 6.25  25    12.5   0.2   6.25   >200   25                         62     1.56 6.25  12.5  3.13   0.39  6.25   100    25                         63     1.56 6.25  6.25  1.56 0.1                                                                             6.25  50     12.5                              64     1.56 6.25  1.56  3.13   0.1   6.25   12.5   3.13                       65     0.78 6.25  3.13  3.13   0.2   3.13   12.5   3.13                       66     0.78 6.25  3.13  3.13   0.1   3.13   12.5   3.13                       67     0.78 3.13  1.56  3.13   0.1   3.13   12.5   3.13                       68     0.78 3.13  1.56  3.13   0.1   3.13   12.5   3.13                       69     0.39 6.25  6.25  6.25   0.2   6.25   50     12.5                       70     1.56 6.25  3.13  3.13   0.1   6.25   12.5   6.25                       71     1.56 6.25  1.56  3.13   0.1   3.13   12.5   3.13                       72     0.78 6.25  3.13  6.25   0.2   6.25   50     12.5                       73     0.78 6.25  6.25  3.13   0.2   6.25   25     6.25                       74     0.78 6.25  1.56  3.13   0.1   3.13   12.5   6.25                       75     1.56 12.5  6.25  3.13   0.39  6.25   25     12.5                       76     0.78 6.25  3.13  3.13   0.2   3.13   12.5   3.13                       77     0.78 6.25  3.13  3.13   0.1   3.13   12.5   3.13                       78     0.78 6.25  3.13  3.13   0.2   3.13   12.5   6.25                       79     0.78 12.5  6.25  3.13   0.2   3.13   12.5   6.25                       80     0.78 6.25  3.13  3.13   0.2   3.13   12.5   3.13                       81     1.56 6.25  1.56  3.13   0.1   3.13   12.5   3.13                       82     0.78 6.25  3.13  1.56   0.1   3.13   12.5   3.13                       83     0.39 6.25  6.25  6.25   0.2   6.25   50     12.5                       84     0.39 6.25  6.25  3.13   0.2   6.25   50     12.5                       85     0.39 6.25  6.25  3.13   0.2   6.25   100    25                         86     0.78 6.25  12.5  6.25   0.39  12.5   50     12.5                       87     0.78 6.25  12.5  6.25   0.78  12.5   50     12.5                       88     0.78 6.25  25    25     0.39  12.5   >200   25                         89     1.56 6.25  3.13  1.56   0.78  6.25   50     6.25                       90     0.78 6.25  12.5  25     0.39  12.5   >200   12.5                       91     0.78 6.25  12.5  12.5   0.39  12.5   50     25                         92     0.78 6.25  25    12.5   0.39  12.5   >200   25                         93     0.78 12.5  12.5  12.5   0.2   12.5   50     25                         94     0.78 12.5  25    12.5   0.78  25     50     25                         95     0.78 12.5  25    12.5   0.78  25     50     25                         96     1.56 6.25  3.13  1.56   0.2   6.25   12.5   6.25                       97     1.56 6.25  1.56  1.56   0.2   6.25   25     6.25                       98     1.56 6.25  1.56  1.56   0.2   6.25   25     6.25                       99     1.56 6.25  6.25  6.25   0.78  12.5   50     12.5                       100    1.56 6.25  3.13  6.25   0.39  6.25   50     6.25                       102    1.56 6.25  3.13  3.13   0.39  6.25   25     12.5                       103    1.56 6.25  3.13  3.13   0.2   6.25   12.5   12.5                       104    1.56 6.25  6.25  3.13   0.39  6.25   12.5   12.5                       105    0.78 6.25  6.25  3.13   0.2   6.25   12.5   12.5                       106    1.56 6.25  3.13  1.56   0.2   6.25   12.5   6.25                       107    1.56 6.25  3.13  1.56   0.1   6.25   12.5   6.25                       108    1.56 12.5  3.13  3.13   0.2   6.25   25     6.25                       109    1.56 12.5  6.25  6.25   0.78  12.5   50     12.5                       110    0.78 6.25  12.5  12.5   0.39  12.5   100    25                         111    0.78 6.25  12.5  6.25   0.39  12.5   100    12.5                       112    0.78 12.5  12.5  6.25   0.78  12.5   100    25                         113    0.39 3.13  12.5  12.5   0.2   6.25   >200   25                         114    0.39 6.25  12.5  12.5   0.39  6.25   >200   25                         115    0.39 6.25  12.5  25     0.78  12.5   >200   25                         116    0.39 3.13  12.5  6.25   0.2   6.25   >200   25                         117    0.39 6.25  12.5  12.5   0.2   6.25   100    25                         118    0.39 6.25  12.5  25     0.39  6.25   >200   25                         119    0.39 6.25  6.25  25     0.39  6.25   >200   25                         120    0.78 6.25  3.13  1.56   0.39  6.25   50     6.25                       122    0.78 6.25  3.13  3.13   0.39  6.25   50     6.25                       123    0.78 6.25  6.25  3.13   0.78  6.25   100    12.5                       124    0.78 6.25  12.5  12.5   0.78  12.5   100    25                         125    0.39 6.25  6.25  6.25   0.1   12.5   50     12.5                       126    0.39 6.25  6.25  12.5   0.2   6.25   100    12.5                       127    0.39 6.25  6.25  6.25   0.2   6.25   50     12.5                       128    0.39 6.25  6.25  6.25   0.39  6.25   50     12.5                       129    0.78 3.13  1.56  1.56   0.1   3.13   12.5   3.13                       130    0.78 3.13  1.56  1.56   0.1   3.13   12.5   3.13                       131    0.78 3.13  1.56  0.78   0.1   3.13   12.5   3.13                       132    0.78 3.13  3.13  1.56   0.1   6.25   25     3.13                       133    0.78 3.13  3.13  1.56   0.1   3.13   25     3.13                       134    0.78 3.13  3.13  1.56   0.1   3.13   25     3.13                       136    0.78 6.25  3.13  3.13   0.78  6.25   100    6.25                       137    1.56 12.5  12.5  12.5   1.56  12.5   100    50                         138    0.78 6.25  3.13  1.56   0.78  6.25   50     6.25                       139    1.56 12.5  12.5  6.25   3.13  12.5   50     50                         140    0.78 6.25  3.13  6.25   1.56  6.25   50     12.5                       141    0.78 6.25  6.25  6.25   0.78  6.25   50     12.5                       142    1.56 6.25  3.13  3.13   0.78  6.25   50     6.25                       143    3.13 12.5  6.25  3.13   0.78  6.25   100    12.5                       144    3.13 12.5  6.25  6.25   1.56  6.25   100    12.5                       145    0.78 6.25  3.13  3.13   0.39  6.25   100    12.5                       146    0.78 12.5  1.56  3.13   1.56  3.13   25     6.25                       147    1.56 12.5  3.13  3.13   1.56  3.13   25     6.25                       148    1.56 12.5  3.13  3.13   1.56  3.13   25     6.25                       149    0.78 6.25  3.13  3.13   0.39  6.25   25     6.25                       150    0.78 6.25  3.13  1.56   0.2   3.13   12.5   6.25                       151    0.78 6.25  1.56  1.56   0.39  6.25   12.5   6.25                       152    0.78 6.25  3.13  1.56   0.2   3.13   12.5   6.25                       153    0.78 6.25  3.13  3.13   0.39  6.25   25     6.25                       154    0.78 12.5  3.13  6.25   0.39  3.13   12.5   6.25                       155    0.78 12.5  3.13  6.25   0.78  3.13   12.5   6.25                       156    1.56 12.5  6.25  6.25   0.78  6.25   50     12.5                       157    1.56 12.5  3.13  3.13   0.2   6.25   25     6.25                       158    0.39 12.5  6.25  1.56   0.39  6.25   25     12.5                       159    0.39 12.5  6.25  3.13   0.78  6.25   50     12.5                       160    0.78 12.5  12.5  6.25   1.56  12.5   50     12.5                       161    0.78 12.5  6.25  3.13   0.39  6.25   50     12.5                       162    1.56 12.5  12.5  6.25   0.78  6.25   50     25                         163    1.56 12.5  12.5  6.25   0.39  6.25   50     25                         164    0.78 6.25  3.13  1.56   0.2   3.13   12.5   3.13                       165    0.78 6.25  3.13  3.13   0.2   3.13   12.5   6.25                       166    0.78 6.25  3.13  1.56   0.2   3.13   12.5   3.13                       167    1.56 6.25  1.56  1.56   0.2   3.13   12.5   3.13                       168    1.56 12.5  12.5  6.25   0.78  12.5   50     25                         169    0.78 6.25  3.13  1.56   0.1   3.13   25     3.13                       170    0.78 6.25  6.25  1.56   0.1   3.13   25     6.25                       171    1.56 12.5  3.13  1.56   0.2   3.13   25     3.13                       172    0.78 12.5  3.13  3.13   0.78  6.25   25     6.25                       173    0.78 12.5  12.5  6.25   0.78  6.25   50     25                         174    0.78 12.5  3.13  1.56   0.39  3.13   12.5   3.13                       175    0.78 12.5  3.13  3.13   0.39  3.13   12.5   6.25                       176    0.39 6.25  6.25  6.25   0.2   12.5   50     12.5                       177    0.39 6.25  6.25  6.25   0.2   12.5   100    12.5                       178    0.78 6.25  3.13  1.56   0.2   3.13   12.5   6.25                       179    0.39 6.25  6.25  6.25   0.39  12.5   50     12.5                       180    0.78 6.25  3.13  1.56   0.1   3.13   12.5   3.13                       181    0.78 6.25  1.56  1.56   0.2   3.13   12.5   6.25                       182    0.78 3.13  12.5  25     0.78  12.5   >200   25                         183    0.78 6.25  6.25  12.5   0.39  6.25   50     12.5                       184    0.78 6.25  6.25  12.5   0.78  6.25   50     12.5                       185    0.78 6.25  6.25  6.25   0.39  6.25   50     12.5                       186    0.78 6.25  6.25  12.5   0.78  12.5   50     12.5                       187    0.78 6.25  6.25  12.5   0.39  6.25   50     12.5                       Known                                                                         Compounds                                                                     CEG    1.56 12.5  12.5  3.13   12.5  >200   >200   50                         CET    0.1  0.78  12.5  0.78   3.13  >200   >200   >200                       CEZ    0.2  1.56  1.56  0.78   6.25  >200   >200   100                         *Staphylococcus aureus FS 289: strain resistant to penicillin                 ##STR491##                                                                    ##STR492##                                                                    ##STR493##                                                               

While the invention has been described in detail and with reference tospecific embodiments thereof, it will be apparent to one skilled in theart that various changes and modifications can be made therein withoutdeparting from the spirit and scope thereof.

What is claimed is:
 1. A compound of the formula, ##STR494## wherein Ais a divalent heteroaromatic ring selected from the group consisting ofcinnoline, naphthyridine, pyrazolopyridine, pyridopyrazine,thiazolopyrimidine, pyridopyrimidine, pyridine, pyrimidine andpyridazine, each of which can be substituted with one or moresubstituents selected from the group consisting of a (C₁ -C₄)alkylgroup, a (C₁ -C₄)alkoxy group, a (C₁ -C₄)alkylthio group, a mercaptogroup, a hydroxy group, a halogen atom, a pyridylsulfonyl group, asulfamoyl group, a carbamoyl group, a (C₁ -C₄)alkylamino group, a di-(C₁-C₄)alkylamino group, a (C₂ -C₆)alkenyl group, a phenyl group and apyridyl group, and wherein the hydroxy group attached to A is ortho tothe amido group also attached to A; R₁ is an amino group, a hydroxylgroup, a hydroxymethyl group or a ureido group; R₂ and R₃, which may bethe same or different, each is a hydrogen atom, a (C₁ -C₄) alkylaminogroup, an amino group, a hydroxyl group, a (C₁ -C₄) alkyl group, a (C₁-C₄)alkoxy group, a chlorine atom, a fluorine atom, an iodine atom, anda hydroxymethyl group and Het is a member selected from the groupconsisting of tetrazol-5-yl, 1,3,4-thiadiazol-5-yl, 1,2,3-triazol-5-yl,1,2,4-triazol-3-yl, 1,3,4-oxadiazol-5-yl, pyridazine-6-yl, each of whichmay be substituted with one substituent selected from the groupconsisting of a (C₁ -C₄)alkyl group, a mercapto group, a hydroxymethylgroup, an aminomethyl group, a (C₁ -C₄)alkylamino group and a hydroxygroup.
 2. The compound according to claim 1, wherein A is pyridine whichmay be substituted with a carboxamido)-α1-C₄)alkyl group; R₁ is ahydroxyl group; R₂ and R₃ are a hydrogen atom; and Het is1-methyltetrazol-5-yl.
 3. The compound according to claim 1, wherein R₁is a hydroxyl group.
 4. The compound according to claim 1, wherein R₁ isa hydroxymethyl group.
 5. The compound according to claim 1, wherein R₁is an amino group.
 6. The compound according to claim 1, wherein R₁ isan ureido group.
 7. The compound according to claim 1 chosen from thegroup consistingof7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(2-methyl-1,3,4-thiadiazol-5-yl-thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(2-methyl-1,3,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(2-methyl-1,3,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyrimidine-5-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1,3,4-thiadiazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1,3,4-thiadiazol-2-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1-methyl-tetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(2-mercapto-1,3,4-thiadiazol-5-ylthiomethyl)-3-cepham-4-carboxylic acid,7-[D-α-(4-hydroxy-7-methyl-1,8-naphthyridine-3-carboxamido)-α-(p-ureidophenyl) acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-7-methyl-1,8-naphthyridine-3-carboxamido)-α-p-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-ureidophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl-3-cephem-4-carboxylic acid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-ureidophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-ureidophenyl) acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α -(m-ureidophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-ureidophenyl-acetamido]-3-(3-hydroxypyridazin-6-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α -(m-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(6-methoxy-4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-ureidophenyl) acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-methylmercapto-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(m-ureidophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-methylmercapto-8-hydroxypyride[2,3-b]-pyrazine-7-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α -(2,3-dimethyl-8-hydroxypyrido [2,3-b]pyrazine-7-carboxamido)-α -(m-ureidophenyl)acetamido]-3-(1-methyltetrazol-5yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α -(4-hydroxypyridine-3-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-ureidophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl-3-cephem-4-carboxylic acid,7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-m-ureidophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(2-hydroxypyridine-3-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxyamido)-α-(p-aminophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α -(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-aminophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1,3,4-thiadiazol-2-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(3-hydroxypridazine-4-carboxamido)-α -(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyrimidine-5-carboxamido)-α -(p-aminophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(7-methyl-4-hydroxy-1,8-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(2-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1,3,4-thiadiazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl) acetamido]-3-(3-hydroxypyridazine-6-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl-3-cephem-4-carboxylic acid, 7-[D-α-(6-methoxy-4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-methylmercapto-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-α-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(5,8-dihydro-5H-8-oxopyrido[3,2-d]pyrimidine-7-carboxamido)-.alpha.-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(2-methylthio-5,8-dihydro-5-oxopyrido[2,3-d]pyrimidine-6-caboxamido)-α -(m-aminophenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(m-aminophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4,8-dihydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4,6-dihydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl) acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-methylamino-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid, [D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-aminomethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(2,3-dihydro-2-oxo-1,3,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-aminophenyl)acetamido]-3-(2-methyltetrazol-5-yl thiomethyl-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(m-aminophenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(3-hydroxypyridazine-4-carboxamido-α-(m-aminophenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(m-aminophenyl)acetamido]-3-(3-hydroxymethylpyridazin-6-ylthiomethyl-3-cephem-4-carboxylic acid,7-[α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[α-(3-hydroxypyridazine-4-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(4-hydroxypyrimidine-5-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[α-(4-hydroxypyrimidine-5-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1,3,4-thiadiazol-2-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl-3-cephem-4-carboxylicacid,7-[α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[α-(7-methyl-4-hydroxy-1,8-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(3-methylthio-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(2,3-dimethyl-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[α-(6-methoxy-4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-hydroxymethylphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(5,8-dihydro-5H-8-oxopyrido[3,2-d]pyrimidine-7-carboxamido)-.alpha.-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[α-(2-methylthio-5,8-dihydro-5-oxopyrido[2,3-d]pyrimidine-6-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[α-(2-hydroxypyridine-3-carboxamido)-α-(p-hydroxymethylphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid, 7-[D-α-(3-hydroxypyridazine-4-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1,3,4-thiadiazol-2-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)-acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3-chloro-4-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(2-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1,2,3-triazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(3-methylthio-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(3-pyrrolidino-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(2,3-dimethyl-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3-chloro-4-hydroxyphenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(3-fluoro-4-hydroxyphenyl)acetamido]-3-(2,3-dihydro-2-oxo-1,3,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-methylamino-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-methylthio-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-hydroxphenyl)acetamido]-3-(5-aminomethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(5,8-dihydro-5H-8-oxopyrido[3,2-d]pyrimidine-7-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(5,8-dihydro-5H-8-oxopyrido[3,2-d]pyrimidine-7-carboxamido)-.alpha.-(m-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(2-dimethylamino-5,8-dihydro-5-oxopyrido[2,3-d]pyrimidine-6-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3-methoxy-4-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(2-methylthio-4,5-dihydroxypyrido[2,3-d]pyrimidine-6-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3-methyl-4-hydroxyphenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(2-methylthio-5-8-dihydro-5-oxopyrido[2,3-d]pyrimidine-6-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(2-piperazino-5,8-dihydro-5-oxopyrido[2,3-d]pyrimidine-6-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(6-methoxy-4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(6-methoxy-4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(6-dimethylamino-4-hydroxy-1,5-naphthyridine-3-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(7-methyl-4-hydroxy-1,8-naphthyridine-3-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(3-hydroxypyridazin-6-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(1,3,4-thiadiazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(3-methoxy-8-hydroxypyrido[2,3-b]pyrazine-7-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-]D-α-(4,6-dihydroxy-1,5-naphthyridine-3-carboxyamido]-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4,8-dihydroxy-1,5-naphthyridine-3-carboxamido]-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(3-hydroxypyridazin-6-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3-chloro-4-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid,7-[D-α-(4-hydroxy-1,5-naphthyridine-3-carboxamido)-α-(m-hydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(5-methyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(5-hydroxymethyl-1,3,4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid, 7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3,4-dihydroxyphenyl)acetamido]-3-(1,2,3-triazol-5-yl thiomethyl)-3-cephem-4-carboxylic acid,7-[D-α-(4-hydroxypyridine-3-carboxamido)-α-(3-fluoro-4-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 8. The compound according to claim 1 having the structure##STR495##
 9. The compound according to claim 1 having the structure##STR496##
 10. The compound according to claim 1 having the structure##STR497##
 11. The compound according to claim 1 having the structure##STR498##
 12. The compound according to claim 1 having the structure##STR499##
 13. The compound7-[D-α-(7-methylthio-4-hydroxypyrimido[4,5-c]pyridazine-3-carboxamido]-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 14. The compound7-[D-α-(4-hydroxythieno[2,3-b]pyridine-5-carboxamido)-α-(m-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 15. The compound7-[D-α-(4-hydroxythieno[2,3-b]pyridine-5-carboxyamido)-α-(m-aminophenylacetamido]-3-(2-methyl-1,3,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid.
 16. The compound7-[D-α-(4-hydroxythieno[2,3-b]pyridine-5-carboxamido)-α-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 17. The compound7-[D-α-(4,7-dihydro-7-oxothiazolo[5,4-b]pyridine-6-carboxamido)-.alpha.-(m-ureidophenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 18. The compound7-[D-α-(4,7-dihydro-7-oxothiazolo[5,4-b]pyridine-6-carboxamido)-.alpha.-(p-hydroxyphenyl)acetamido]-3-(1-methyltetrazol-5-yl thiomethyl)-3-cephem-4-carboxylicacid.
 19. An antibacterial composition which comprises a therapeuticallyeffective amount of a compound of claim 1 or a non-toxicpharmaceutically acceptable salt thereof and a pharmaceuticallyacceptable carrier or diluent.
 20. A method of treating a patientsuffering from a bacterial infection which comprises administering thecomposition of claim 1 to said patient.